22 October 1958 REPORT ON K>2p-302 These experiments were carried out (a) to determine if K>2p-30>2p;>2p' and a crude preparation of a fungus wo>2p,>2p41d induce psychic changes in mans >1p(b>1p) to determine the dose range necessary to elicit a reaction, and >1p(c>1p) to obtain Information on the clinical charac>1p- teristics of the reaction. 2 >2pm>2piet>2ps>2p,>2pl Eleven Negro male former addicts who were in good physical and mental health served as. subjects. All these patients were familiar with the effects of m>2par>2pi>2phuana>2p, LSD, and were accus>1p- tomed to the experimental environment. General Conditions: All experiments were conducted 'in a special ward. Men entered the ward on the night preceding the test. They were awakened at >1p'>2po a>1p.>2pm2>2p. Drugs were given>1p.orally with the patients fasting. Patients were observed after >1p1>1p0 m>2pinutes rest in bed but-were free to mingle with other p>2patients during intervals between observations. Measurements.* The following measurements were obtained at hourly intervals once before, and eight ti>2pnes after administra>1p- tlon of the drug: >1p1>1p. rectal temperature-; 2>1p. systolic and d2iastolic blood pressure; 3>1p. pulse rate; 4>1p. respiratory rate; >1p5>1p. pupillary size; 6>1p. threshold for k>2pree>1pjerk>1p; 7>1p. LSD questionnaire; >2pPa >2pge >2p->2pR>2pe>2p-oort an' >2pK>2p->2p'>2p1>1p02 notes on behavior. Dr>2p-u>2pgs>2po>1p. K>1p-30>2p2>1p- was dissolved in 3>1p-5 >2pc>2pc>2p. of ethyl alcohol, and diluted in distilled water to 30 cc. The crude fungus was dispersed in water, using a Waring blender. Placebo was spinach ground >2pi>1p-n water in a blender to>2p'simula>1p2te the appearance of the crude fungus. Doses used are sho>2pl>2pdn in the-tables attached which also give a qualitative assessment of the results on the various measurem>2p4>2p.>2pn>2p*>2p,>2p,>2ps>2p. In addition, data on patients receiving 2>2p.5 gra>2pms or more of >2pK>2p-302 >1p(4>1p)>2p. or 15>2p.0>2p-20 grams of fungus >1p(>1p5>1p) were analyzed and averaged according to the>2p'system used in assessing LSD effects. 2 Results>1p: >2pK>2p-302>1p. Pup>2pillary dilatation was note d in every case even with a dose of 0>2p.3 grams. Change in-blood pressure and knee jerks did not seem significant until 2>1p.5>1p-3>1p.0 grams were given. Subj>2p-ec>2pl>2pi>2p.>2pive effects were not noted cons>2pisten>2p,>2p%>2pIly until 2>2p.5>1p-3>1p.0 grams were given. All >2p4 patients receiving these a>2pmounts became elated., silly., and giggled. They reported nervousnes2s, vague fears, changes in body sensation (numbness, light>2pi etc*)* One (Foster) gave a picture very similar to LSD (euphoria, silliness.. visual distortion, depersonalization>2p.>2p, lights>1p- colors., etc.). Crude Fungus. Definite changes in blood pressure, pupila and k>2pneejerks were observed with doses of 10>2p-20 grams. All patients receiving 15>2p-20 grams had subjective effects, most frequently described as 1being those of marihuana. The LSD patt>2pe>2pl>2p-n (anxiety, perceptual distortion., h>2p->2p->2p->2pIlucinations>1p) were seen in 3>1p. Page on Placebo. All results were negative. C>2pC>2p)>2pN>2pP4>2pP>2p-NTS The results warrant the statement that both the crude fungus and >2pK>2p->2p"02 have symp>1p'athomi>2pmetic activity and will induce psychic changes in man. The data are not sufficient to characterize the reaction completely or to assess with any exactness the relati2ve potencies, About 2>2p.5>1p->2p@>2p.0 grams of >1pK>2p-302 seems roughly equivalent to >1p1>1p0>1p->1p1>1p5 grams of the crude >2pmaterial>1p. Plans: See letter. 0 2 -a n Dose pur) i 1 BP KJ Subjec tive 0 0 "DIL'ferent" 0.3 + + 0 0-.4 2 0 0.5 + + + Relaxed., nausea 0.6 0 +. 0 0.7 0 0 0 1.0 0 + 0 2 1.25 Nervou's.,.keen hetring.,, etc. 4,5 0 + 0 2.0 0 0 0 2.0 + 0 0 2 2.5' Nervous 3;0 + Nervous + Euphoric, sill@ 2.5 I L SD- 1 i ],.e 21. Nervous, skin sensitive Systolic blood pressure BP - KJ - Threshold for kneejerk + - Indicates that measurement judged positive by inspection Crude Fungus >2pMan Dose-Grams >2pP>2pu>2pD>2pi>2pl BP >2pK>2pi Subjective >1p5>2p.>1p0 >1p+ >1p+ >1p+ Relaxed., d>2pifferen>1p- >1p1>1p0>1p.>1p0 >1p0 Giggles, arms hea>2p, >1p1>1p5>2p->1p0 2 elated., no hallu>2p- c>2pinations >1p+ Relaxed., logs >2pnu>2pr>2p,>2p.>2p@ >1p1>1p5>1p.>1p0 >2p1>1p5>1p.>1p0 >1p0 Relaxed>2p.>2p.>2p'drowsy>2pt LSD-like. 2 "reefer. 20>1p.0 >1p+>2pc >2pZ>2p@>2p,>2pTervous>1p, l>1pights>1pr LSD-like LSD-like 20>1p.0 placebo Spinach >1p0 >1p0 >2p@>82po >1p0 >1p0 >1p0 >1p0 >1p0 Rion >1p0 >1p0 >1p0 >2pm >1p0 >1p0 >1p0 ?a 15>2p-20 >1p-CEIVINC>2pR 2>2p.>2p'>2p@>2p,>2p-3>2p-0 >1pr>2pj>2pD>2pA>1pMS >2pK>1p->2p102>2pi AVE>2pRA>2pGE C>2pl>2piANCTES IN PATIENTS R>2pz >2pG>2pI>2pR>2pMS OF CR>2pUD>2pE >2pF>2pI>2pJ>2p1>2p4>2pC>2p.>2p,>2pUS A>2pN>2pq>1p) PLACEB>2pO>2p. 2 >2px >2px systolic >2px Nu>2pmber pupillary Blood Knee Number Drug Patients' Diameter pres>2p-sure Jer>1pR Questions K >1p0 2 >1p+ 7>1p.6 >1p+ >2p69 >1p+ 9>1p.1 >1p+ >1p8>1p.>2p0 48 Crude Fungus 0 >1p5 >1p+ 9>2p.5 >1p+ >1p5>1p1 >1p+ >2p4>1p.3 >1p+ 3>2p.0 >2p:>2p>>1p.5 >1p0 Placebo 4 >1p1 mcg>1p.>1p/kg>1p. 6 >1p+ 17 >1p+ 93 >1p9>1p.2 >1p5>1p0 LSD, X Represents areas under time action curves.