09
                             'APTED BLOCKING Of LSD REACTICN
                        ATTE                                  BY
                                  RETREA17A[NT VITH SOL
                 Purooset  To datermlnc if three days   pretreatment   wifh
           SOL  would attenuate the LSD reaction*
                 Background:  Ginzet and 2&layer-Gross have reporfed   that
           fwa days prefrtatme  nt-with 3 mg. of 80Lplus  an  addlt.tonal  i  m
           an  the day of the test, atfenuated or completely blocked the
           reaction lftduced by sma.11 doses 140 to 50 mcgm.) of LSD.  We
           atte@pted to reproduce  their  experiment*,  using  doses  of  1-1.5
           MCGI.10 of LSD, but could show  no  significant  blocking.  Four  of
           the 8 pat-tenfs used, however, had very  minor  rca:cflons  to  LSD.
           The experiment, therefore, was repeated using larger doses of
           LSD In order to Insure a fairly marked degree of reaction.
                                        METI@-MS
                            Ten Negro male postaddlctss              i had been
                                                          all of whor,
           ab'stinent from opiates for.at least  six  months.,  voluntecred  as
            --b      f 0,- "$Lc           a!; were in good menial and phys teal
           condition,, alid'all had expericneed LSD-affec+s prevlousfy.
                               1                                         T    -L
                  f. i 7 Prucs  were all given  lr, solution.  Ciia mg. c' 'OU
           was administered at   8 a.m. a.-id 6 p.m. for three days prior to
           -.the LSD. C)n the morning of the test I mg.  of  BOL  was  given  at

               6 otclock,    and LSD was given            9 0 clock.       T,ic doze of LSD
               was  1.5 to   3 mct-                                                   to Induce
                                   .,M. ./k 9, LSD doses were chosen so           as
                   I t a S t S. "Gra<fil 3' reac t I Oft_T t ha I JUCInatlans la  all s2ubjects),
                     Destgng   A randomized double-blind design                was    used.     Each
               patient recolved the foltowlng tr.eafmentst   (1) SOL placebo
               plus LSD, and (2) BOL plus LSD*   The combtnaflofts of SOL@placebo
               plus-LSD@placebo, and SOL plus LSD placebo were no2t s.tudied,
               p&rtty because      of shortage of naterial and partly because.
               previous experiments have shovn that anty Inconsequ                  gntial effects
               would.be expgflted.
                     Observattonst   Kneelerks          were asscssc<i by      deferm.Inlng      the
          2     angle f hrough ,vhich a cal lbrated harm-nor must fa It. in order to
               ellett barely perceptible knielerks#   Systolic blood pressure
               was meauured by auscultatory'mefhod.   Pu.-311tary dlamefer wa&
             ,.,.measured In   a   dark   room   under   constant   conditions    of    light    and
               accommodations.      2     These observatl6ns    were     obtained     twicz     before
               and at  Intervals      of   I., 2-t/2,-4,   5-1/2,   and   7   @tours   afte.-    LSD,
                     A mod I f I ca t I ort of @t Pe i5bramson'Jarvlk quest loinatrt.- was
               administered       by the aide    f-d2ice before and      af 1/2   1-1/2   3
               4-11@2$  6.,  and  i3 .,ours  affer   LSD. -'"Cllnlc4l-  rades' were asslgned
               On  the   @@asls   of sh or   mental  s@i3fus    extininafl-on n.crfor,.-,et.]
               Intervals       after LSD.

               Analysis of Data,.-  Areas of the time-action curve were
          Calculated In the usual wav%and means and sfa,ndard errors
          txfracfgd from the raw data.  Questions were counted
          gllmlnaflng any answers that were also reported positive prior
          fo administration of LSD.
               Resultst  Resutfs2 are shown In the  tablew  There appears
          fo be-'& slight trend toward the rcductton of all aspects of
          the LSD-reaction. The differences, however  are   slight aad
          not $I AffIcents all being well within the  error of meavure.,.nent.
               Conclusiont  It was concluded that admlnlstratton of 2 mo.
          of BOL daily for3 three days prior to ad-,iintstra-flon of the LSD
          will not block the response to 1.5 to 3 mcgm./ka. of LSD*

                 ATTEtl."TED CLOCII"Ilt.-IO Of ..TI-IE LSD Ri"t,@CTICt-4 BY DAYS
                       P.D.ET,?.,".AriiEt.'.T t;l iH I L. C  S' LTZ'l C'-' DA I L Y
      MEASU.RE                               S L ?LTCEBO &  L$3      ML 4- LSD
      Pate2llar Reflex                       4*34 4. 0.3              4.48 t ,",.46
      Pvpiilary  Size                         9.47 4. 0.53           7.61     1. 06
      Systolic  Blood  Pressure               5.89  0.712            4.38  4@ 3. j9
      Nunber of positive Ansvsers               76 4. It                62 4   13
      Crade
             7                                 .7
                  Figur'-s are nieant-cn 10 sv6jecfs      standard errors.